En route to photoaffinity labeling of the bacterial lectin FimH

• Thisbe K. Lindhorst,
• Michaela Märten,
• Andreas Fuchs and
• Stefan D. Knight

Beilstein J. Org. Chem. 2010, 6, 810–822, doi:10.3762/bjoc.6.91

• shown both by MS/MS studies and by affino dot–blot analysis. Keywords: diazirines; FimH; lectins; MS/MS analysis; photoactive mannoside ligands; photoaffinity labeling; Introduction Photoaffinity labeling is a technique by which ligand binding sites of a receptor protein can be identified in solution

• photoactive mannoside ligands 2, 12, and 13. Assignment of peptide fragment ion pattern according to Biemann [25]; left two columns: angiotensin; right two columns photo-crosslinked product of angiotensin and diazirine 2 (Figure 4). Acknowledgements We are thankful to PD Dr. Buko Lindner (Research Center